Fig. 2: ErbB4 JM-a and JM-b selectively activate different STATs.

a, b Phosphorylation status of STAT5a and STAT5b (activating phosphorylation on Y694/699) in MDA-MB-468 cells expressing ErbB4 JM-a or JM-b. Representative blots of n = 7 (STAT5a) and n = 8 (STAT5b) independent experiments. c, d Densitometric quantification of STAT5 Western analyses, such as shown in panels a and b, from experiments with the indicated cell lines. COS7: n = 4 (STAT5a) and n = 6 (STAT5b) independent experiments. HC11: n = 4 (STAT5a and STAT5b) independent experiments. MCF-7: n = 5 (STAT5a and STAT5b) independent experiments. MDA-MB-468: n = 7 (STAT5a) and n = 8 (STAT5b) independent experiments. Kruskal Wallis ANOVA. Benjamini, Krieger and Yekutieli adjusted P-values. e–h Proximity ligation assays (PLA) of association of ErbB4 JM-a or JM-b with STAT5a or STAT5b in MDA-MB-468 cells. Quantification of the data is shown in panels f and h. n = 13–16 cells examined over two independent experiments. Brown Forsythe and Welch ANOVA and Dunnet’s multicomparison test. i–l Immunofluorescence analysis of nuclear localization of the STAT5 subtypes in HC11 cells expressing ErbB4 JM-a or JM-b after NRG-1 stimulation. Confocal microscopy images (i, k) and quantification of the co-localization of STAT5 signal and the chromatin stain DAPI (j, l) are shown. n = 36 (STAT5a) and n = 41 (STAT5b) examined cells over two independent experiments. Two-tailed Mann–Whitney U test. In the boxplots the line represents the median, the box the interquartile range, and the whiskers the whole range of values. Source data are provided as a Source Data file.