Fig. 5: IVF of MII oocytes and development of zygotes. | Nature Communications

Fig. 5: IVF of MII oocytes and development of zygotes.

From: Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

Fig. 5

a Representative images of the development of oocytes in vitro (from MII oocytes to the blastocyst stage). Each experiment was repeated three times with similar results, and representative images are shown. b IVF analysis of oocytes. Pronuclear formation rate: Percentage of 2-pronuclei to total number of oocytes involved in IVF. c Evaluation of the embryonic developmental performance. W/O VTF: PAFs were only encapsulated in hydrogel and did not undergo vitrification; W/ MIH + LIH: PAFs encapsulated in hydrogel were warmed with MIH and LIH after vitrification; Fresh oocytes: MII oocytes obtained directly from the ampulla of the oviduct of a mouse; One-way analysis of variance (ANOVA) and Tukey’s post hoc were used for statistical analysis (b); Two-way analysis of variance (ANOVA) and Tukey’s post hoc were used for statistical analysis (c). ns: p > 0.05. n = 10–20 for five replicates (b); n = 10–20 for three replicates (c). n: number of follicles used in each experiment. Data are presented as the mean ± SD (b, c).

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