Fig. 7: Birth of offspring after transplantation of 2-cell stage zygotes. | Nature Communications

Fig. 7: Birth of offspring after transplantation of 2-cell stage zygotes.

From: Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

Fig. 7

a Mouse pups were born after transplantation of 2-cell stage embryos. b Analysis of the mouse birth rate. c Reproductive performance after zygote transfer. d Birth of the second generation of mice. e Reproduction performance of the first generation of mice. W/O VTF: PAFs were only encapsulated in hydrogel and did not undergo vitrification; W/ MIH + LIH: PAFs encapsulated in hydrogel were warmed with MIH and LIH after vitrification. Fresh oocytes: MII oocytes obtained directly from the ampulla of the oviduct of a mouse; One-way analysis of variance (ANOVA) and Tukey’s post hoc were used for statistical analysis (b). ns: p > 0.05. n = 10–20 for four replicates (b). n: number of follicles used in each experiment. Data are presented as the mean ± SD (b).

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