Fig. 6: Synergistic effects of TEAD and AKT inhibitors combination in cancer cells.

a Drug combination experiments using MGH-CP1 and AKT inhibitor ipatasertib, and Heatmaps show color-coding as percentage of cell viability normalized to untreated controls. Heatmaps of Bliss score for MGH-CP1 and ipatasertib combination were shown. b Representative images in 3D tumor cultures of HUTU80 cells treated with MGH-CP1, MGH-CP12, ipatasertib or combination. Colony diameters were measured to assess the tumor growth with inhibitors (n = 51, 53, 59, 33, 44, 75 colonies for control, CP1, CP12, ipatasertib, CP1 with ipatasertib, and CP12 with ipatasertib, respectively at day 4, n = 104, 94, 75, 97, 74, 77 colonies for the same sample order as above at day 8). Colony numbers were determined (n = 3 biological repeats). Scale bar, 200 µm. c Representative images in 3D tumor cultures of HCT116 cells treated with MGH-CP1, MGH-CP12, ipatasertib or combination. Colony diameters were measured to assess the tumor growth with inhibitors (n = 52, 58, 66, 45, 67, 69 colonies at day 4, n = 68, 79, 85, 58, 66, 84 colonies at day 11, for the same sample order as in b. Colony numbers were determined (n = 3 biological repeats). Scale bar, 200 µm. d Representative images of fluorescent staining with Calcein-AM and Propidium Iodide in Huh7 and HUTU80 cells treated with MGH-CP1, ipatasertib and combination. Cell death percentages were determined by dead cell/total cells (n = 3 biological repeats). Scale bar, 200 µm. e 7-AAD exclusion assay showing the histogram of 7-AAD staining in MDA-MB-231, DLD1 cells treated with MGH-CP1, ipatasertib and combination (representative histograms were chosen from n = 3 biological repeats). Data are represented as mean ± S.E.M. P values were determined using two-tailed t-tests. Source data are provided as a Source Data file.