Fig. 1: Single-cell transcriptome analysis of thoracic and lumbar proprioceptors at e15.5.
From: Molecular identity of proprioceptor subtypes innervating different muscle groups in mice
a Schematic illustrating central and peripheral connectivity of e15.5 proprioceptors at thoracic (left) and lumbar (right) spinal levels. Ab-pSN, abdominal muscles-connecting proprioceptors; Ba-pSN, back muscles-connecting proprioceptors; Li-pSN, hindlimb muscles-connecting proprioceptors; MMC, median motor column; HMC, hypaxial motor column; LMC, lateral motor column. b UMAP visualization of tdTomato+ neuron clusters from PvtdTom embryos at e15.5. c Gene expression analysis (logcounts) of proprioceptors (Pv, Runx3, Etv1, Ntrk3), mechanoreceptors (Ntrk2, Maf), postmitotic neurons (Avil, Isl1), proliferating neurons (Mki67, Mcm2, Pcna) and glial (Mpz, Apoe) markers. d UMAP visualization of proprioceptor clusters identified from analysis of cluster 1. e UMAP visualization of proprioceptor clusters color-coded according to the thoracic (red) and lumbar (blue) origin of the cells. f Percentage of proprioceptors originating from lumbar (left) and thoracic (right) DRG in different proprioceptor clusters. g Differential gene expression analysis (logcounts) in proprioceptive clusters (pS1, green; pS2, dark blue; pS3, orange; pS4, light blue; pS5, yellow; pS6, red; pS7, blue). h Representative single molecule fluorescent in situ hybridization (smFISH) images of thoracic (top) and lumbar (bottom) e15.5 DRG sections showing proprioceptors (Runx3+; Pv+) expressing Trpv1. Scale bar: 25 µm. i Percentage of proprioceptors (Runx3+; Pv+) expressing Trpv1 in thoracic and lumbar DRG at e15.5 (mean ± SEM, n = 3 animals). Source data are provided as a Source Data file.
