Fig. 2: Ligands bind to the conformationally variable site on BTB domain.
From: Increased slow dynamics defines ligandability of BTB domains
a Mapping of the chemical shift perturbations of methyl groups (upper row) and backbone amides (lower row) resulting from the binding of 2GG4 to MIZ1BTB. The crystal structure is shown in “open” (PDB id: 2Q81:A) and “closed” (PDB id: 2Q81:B) conformations. All methyl-containing residues (ILVAMT) are shown in green with sphere radii corresponding to the magnitude of chemical shift perturbation. Perturbations of backbone amides are in the dark-blue to light-blue colors corresponding to their CSPN/H effect. CSP effects correspond to the MIZ1BTB:2GG4 molar ratio of 1:4. b Mapping of the amide chemical shift perturbations CSPN/H upon binding of the three fragments onto the crystal structure of MIZ1BTB with bound HUWE1 (orange) (PDB id: 7AZX).
