Fig. 4: Inhibition of ectoTLR3 clustering by a blocking antibody.
From: TLR3 forms a highly organized cluster when bound to a poly(I:C) RNA ligand
a Representative micrograph (n = 1116) of the ectoTLR3-mAb12-poly(I:C) complex. An scFv form of the antibody mAb12 is used for the complex formation. The TLR3s in the clustered (middle) and dimeric states (right) are marked with green circles. The 2D class averaged images of TLR3 are shown above. The molar ratio of mAb12 vs. TLR3 was 0.5:1. b Atomic model of the ectoTLR3-mAb12-poly(I:C) complex. TLR3-dependent IFN-β (c) and NF-κB (d) assays. TLR3 was stimulated by poly(I:C) (gray). Reporter signal inhibition by antibody treatment in a dose-dependent fashion is shown (blue). The cells were pretreated with the antibody one hour before poly(I:C) treatment. N = 4 (c) and n = 3 (d) independent experiments were performed. Data are presented as mean values ± SEM. Two-tailed t test with Welch’s correction (c) and two-tailed t test without correction (d) were used to calculate p values. ns; not significant *; p < 0.05, **; p < 0.01; ***; p < 0.001, ****; p < 0.0001. Source data and p values are provided as a Source Data file.
