Fig. 5: Interaction between TLR3 dimers in the cluster.
From: TLR3 forms a highly organized cluster when bound to a poly(I:C) RNA ligand
a The charge distribution of the TLR3s in the clustered state. The negatively charged and positively charged surfaces are colored in red and blue, respectively. b A representative micrograph of the NT-mut mutant (K117D, K139D, K145D). The number of TLR3s in the clustered state is significantly reduced. Representative micrograph is from an n = 1113 cryo-EM experiment set. c A representative micrograph of the CT-mut mutant (D523K, D524K, E527K). The mutation practically eliminates the number of TLR3s in the clustered state. Representative micrograph is from an n = 2010 cryo-EM experiment set. d Structural comparison of wild-type TLR3 against the two charged-reverted mutants. The r.m.s.d. of the NT-mut and CT-mut TLR3s aligned against the wild-type TLR3 is 0.279 Å and 0.147 Å, respectively.
