Table 1 Comparison of EGFR mutation detection in surgical specimens (tumors) from NSCLC patients using different methods

From: A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutations

Sample code

Sex

Stage (AJCC)a

Sanger

EGFR(+)HX103(+) (%)b

EGFR(+) (%)c

HX103(+) (%)d

#1

M

I

19del

+ (37.7)

+ (72.7)

+ (39.7)

#2

M

I

+ (62.2)

+ (98.9)

+ (58.0)

#3

M

II

19del

– (22.2)

+ (93.9)

– (21.9)

#4

F

II

L858R

+ (56.2)

+ (89.9)

+ (56.5)

#5

M

I

19del

+ (40.6)

+ (85.2)

+ (39.0)

#6

F

I

19del

+ (60.9)

+ (90.5)

+ (61.8)

#7

F

I

L858R

– (1.69)

– (11.2)

– (2.40)

#8

M

I

– (22.6)

– (21.6)

+ (90.6)

#9

F

II

e

+ (44.4)

+ (59.3)

+ (54.6)

#10

F

II

– (5.35)

– (29.3)

– (13.4)

#11

M

II

– (2.39)

– (28.3)

– (4.48)

#12

F

I

L858R

+ (98.7)

+ (98.9)

+ (99.6)

#13

M

III

L858R

+ (80.3)

+ (98.2)

+ (65.7)

#14

F

IV

– (0.02)

– (14.7)

– (0.50)

#15

F

I

– (0.03)

– (0.26)

– (9.84)

#16

F

II

– (0.15)

– (0.78)

– (1.72)

#17

F

I

L858R

+ (57.8)

+ (64.8)

+ (74.7)

#18

M

I

– (1.70)

– (2.52)

– (20.1)

#19

F

I

– (0.04)

– (5.18)

– (2.48)

#20

F

II

L858R

+ (39.8)

+ (43.2)

+ (50.0)

#21

F

II

– (7.26)

– (13.9)

– (22.9)

#22

M

I

L858R

+ (59.9)

+ (65.1)

+ (76.4)

#23

F

I

L858R

+ (79.8)

+ (79.8)

+ (97.5)

  1. aAJCC (American Joint Committee on Cancer).
  2. bFor EGFR(+)HX103(+), the threshold value of 30.1% was used to differentiate EGFR-activating mutations.
  3. cFor EGFR(+), the threshold value of 36.3% was used to differentiate EGFR-activating mutations.
  4. dFor HX103(+), the threshold value of 31.0% was used to differentiate EGFR-activating mutations. “–” represents no mutations or mutation negative; “+” represents mutation positive.
  5. eDroplet digital PCR assay showing EGFR-activating mutation positive (EGFR 19del).