Fig. 4: SLR1 confers resistance to RSV infection in rice. | Nature Communications

Fig. 4: SLR1 confers resistance to RSV infection in rice.

From: Independently evolved viral effectors convergently suppress DELLA protein SLR1-mediated broad-spectrum antiviral immunity in rice

Fig. 4

a Plants and leaves of LS, SLR1-GFP and RNAi-SLR1 20 d after inoculation with RSV. The areas of typical yellow stripes and curl or death of the young leaves represent the degree of disease symptoms. Values were obtained from n = 30 biologically independent plants, n = 3 biologically independent replicates per genotype. Scale bars = 6 cm (upper panel) and 1 cm (lower panel). b Disease incidence and grades of symptoms in LS, SLR1-GFP and RNAi-SLR1 20 d after inoculation with RSV. c Results of qRT-PCR showing the relative mRNA levels of RSV CP in RSV-infected LS, SLR1-GFP and RNAi-SLR1 rice plants. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. d The accumulation of RSV CP protein in RSV-infected LS, SLR1-GFP and RNAi-SLR1 rice plants by western blot. RbcL serves as the loading control. e Plants and leaves of SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox 20 d after inoculation with RSV. The areas of typical yellow stripes and curl or death (highlighted as white arrowheads) of the young leaves represent the degree of disease symptoms. Values were obtained from n = 30 biologically independent plants, n = 3 biologically independent replicates per genotype. Scale bars = 6 cm (upper panel) and 1 cm (lower panel). f Disease incidence and grades of symptoms in transgenic rice plants SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox 20 d after inoculation with RSV. g Results of qRT-PCR showing the relative expression levels of RSV CP in RSV-infected SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox rice plants. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. h Western blot showing the accumulation of RSV CP protein in RSV-infected SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox rice plants. RbcL serves as the loading control. Source data including uncropped scans of gels (d and h) and p values of statistic tests (c and g) are provided in the Source data file.

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