Fig. 1: Delineating the ETV6-RUNX1 regulome in childhood B-ALL. | Nature Communications

Fig. 1: Delineating the ETV6-RUNX1 regulome in childhood B-ALL.

From: Regulome analysis in B-acute lymphoblastic leukemia exposes Core Binding Factor addiction as a therapeutic vulnerability

Fig. 1: Delineating the ETV6-RUNX1 regulome in childhood B-ALL.

a Integrative Genomics Viewer (IGV) screenshot of ETV6-RUNX1 binding at the IER2 locus in an ETV6-RUNX1+ cell line (Reh, upper panel, red dashed line) or patient-derived ETV6-RUNX1+ leukemia samples (bottom panel, blue dashed line). HPA and PAS, two independent ETV6 antibodies; DNaseI, DNaseI hypersensitivity sequencing; control, signal following immunoprecipitation with an IgG antibody. b MEME enrichment of motifs in sites bound by ETV6-RUNX1. c Correlation matrix for normalized signal across all ETV6-RUNX1 binding sites. d Distribution of peaks relative to their nearest genes (see methods for analysis). e ETV6-RUNX1 binding across genes binned according to relative expression in ETV6-RUNX1+ B-ALL as compared to all other subtypes. lfc: log2 Fold Change. One-way Fisher’s exact test revealed significant overrepresentation of ETV6-RUNX1-bound genes in the indicated groups, Benjamini & Hochberg correction for multiple testing applied, only adjusted p values < 0.05 are shown (e). Source data are provided as a Source Data file.

Back to article page