Fig. 2: PD-associated species nominated by consensus of MaAsLin2 and ANCOM-BC.
From: Metagenomics of Parkinson’s disease implicates the gut microbiome in multiple disease mechanisms
Analysis included N = 724 biologically independent samples from 490 PD and 234 neurologically healthy control (NHC) subjects. a 257 species (denoted by circles in the plot) were tested in microbiome-wide association study (MWAS) with two statistical methods: MaAsLin2 and ANCOM-BC. The results are shown according to significance (−log10 of the FDR) achieved by MaAslin2 (Y-axis) vs ANCOM-BC (X-axis). Corresponding untransformed FDR values are provided in parentheses on the X and Y axes for easier interpretation. 84 species were nominated as PD-associated, defined by FDR < 0.05 by one method and FDR≤0.1 by the other: 68 achieved FDR < 0.05 by both methods, 10 achieved ANCOM-BC MaAsLin2 FDR < 0.05 by MaAsLin2 and FDR≤0.1 by ANCOM-BC, and 6 achieved FDR < 0.05 by ANCOM-BC and FDR≤0.1 by MaAsLin2. Blue: abundance is significantly elevated in PD. Red: abundance is significantly reduced in PD. Gray: not significantly associated with PD. Vertical and horizontal dashed lines denote points on X and Y axes that correspond to FDR=0.05. b, c Venn diagrams summarizing the overlap of species detected by MaAsLin2 (dotted circle) and ANCOM-BC (solid circle) at FDR≤0.1 (b), and at FDR < 0.05 (c).
