Fig. 2: Amo, Desl and Map inhibit the signaling cascades downstream of the three UPR pathways. | Nature Communications

Fig. 2: Amo, Desl and Map inhibit the signaling cascades downstream of the three UPR pathways.

From: Maprotiline restores ER homeostasis and rescues neurodegeneration via Histamine Receptor H1 inhibition in retinal ganglion cells

Fig. 2: Amo, Desl and Map inhibit the signaling cascades downstream of the three UPR pathways.The alternative text for this image may have been generated using AI.

a Immunoblot of HEK293T cells showing the effects of the three compounds on the PERK-CHOP pathway. Quantification of phosphorylated PERK (p-PERK), ATF4 and CHOP 24 h after DMSO, Amo, Desl, or Map (10 µM) treatment in the presence of Tm/Tg (1 µM), relative to control (without Tm/Tg). b Immunoblot showing the effects of the three compounds on the ATF6 pathway. Quantification of total ATF6 protein level 24 h after DMSO, Amo, Desl or Map treatment in the presence of Tm/Tg, relative to control (without Tm/Tg). c RT-PCR showing the mRNA levels of un-spliced and spliced forms of XBP-1 (XBP-1u and XBP-1s). Quantification of XBP-1s mRNA level 24 h after DMSO, Amo, Desl, or Map treatment in the presence of Tm/Tg, relative to control (without Tm/Tg). d Immunoblot showing the effects of the three compounds on the IRE1α-XBP-1/JNK pathway. Quantification of XBP-1s, phosphorylated JNK and IRE1α 24 h after DMSO, Amo, Desl, or Map treatment in the presence of Tm/Tg, relative to control (without Tm/Tg). e qPCR mRNA measurement of the UPR genes in HEK293T cells treated for 12 h with DMSO, Amo, Desl, Map (10 µM) in the presence of Tm/Tg (1 µM), relative to control (without Tm/Tg). All data in this figure are presented as means ± s.e.m., n = 3 independent replicates, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source data file.

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