Fig. 3: In vivo application of the three hit compounds inhibits ONC-induced ER stress in RGCs. | Nature Communications

Fig. 3: In vivo application of the three hit compounds inhibits ONC-induced ER stress in RGCs.

From: Maprotiline restores ER homeostasis and rescues neurodegeneration via Histamine Receptor H1 inhibition in retinal ganglion cells

Fig. 3: In vivo application of the three hit compounds inhibits ONC-induced ER stress in RGCs.The alternative text for this image may have been generated using AI.

a Schematic depicting the mouse ONC model and the timeline of ER stress and neuroprotection evaluation. For compound treatment, each eye received intravitreal injection with 2 µl of 2 mM test compounds once and intraperitoneal (i.p.) injection daily (15 mg/kg). b, d, f, h Immunohistochemistry analysis showing the levels of CHOP (b), p-eIF2α (d), ATF4 (f), and p-JNK (h) in GCL (ganglion cell layer) of retina sections at 3dpc. c, e, g, i Quantification of corresponding fluorescence intensities of CHOP (b), p-eIF2α (d), ATF4 (f), and p-JNK (h) in GCL. Data are presented as means ± s.e.m., n = 3 mice, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source data file.

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