Fig. 8: Map effectively inhibits axon injury-induced intracellular Ca²⁺ influx and ER Ca²⁺ release.

a jGCaMP7s expression in HEK293T cells as an indicator of cytoplasmic Ca²⁺ levels. b Quantification of jGCaMP7s fluorescence intensity, 0.5 h after DMSO or Map treatment in the presence of Tm/Tg, represented as fold changes to DMSO-treated control cells. n = 9 independent replicates. c Cytoplasmic Ca²⁺ influx in HEK293T cells immediately after Tm/Tg (1 µM) treatment, n = 3 independent replicates. d In vivo retina Ca²⁺ imaging by SLO in living animals expressing jGCaMP7s in RGCs shows cytoplasmic Ca²⁺ influx in RGCs induced by axon injury. e Quantification of relative intra-RGC Ca²⁺ levels, represented as fold changes to the baseline fluorescence intensity of DMSO-treated retina. n = 5 mice. f Ex vivo measurement of ER Ca²⁺ retention in RGCs expressing D4ER after ONC. The D4ER FRET ratio value (Citrine/ECFP) reflects the steady-state ER Ca²⁺ concentration. g Quantification of ER Ca²⁺ levels in RGCs expressing D4ER, represented as FRET ratios. n = 5 mice. All data in this figure are presented as means ± s.e.m., **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source data file.