Fig. 6: Immune landscape of OS.

A Heatmap of 72 curated immune markers within OS patients. The integrative subtype information of each patient is annotated at the top. B The immune score, stromal infiltration score and ESTIMATE score among each integrative subtype. The statistical analysis was made by Kruskal–Wallis test. C Immunohistochemistry of CD4, CD8, IDO1, FOXP3 and PD-L1 between iCluser 1–2 and iCluster 3–4. D CDR3 clonotypes of TCRs in each subtype. P = 0.0069, the statistical analysis was made by Kruskal–Wallis test. E Distribution of immune subtypes within integrative subtypes. Asterisks define significance levels (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).