Fig. 4: Structural activities and hypotensive effects of bradykinin and BK-based azapeptide analogues.
a Circular Dichroism (CD) spectra of bradykinin-based azapeptides. Bradykinin azapeptide analogues were scanned from 200 to 260 nm on CD spectrometer for 3 repeats per peptide. The CD spectra were plotted by using the average value of three repeats. b Hemodynamic effects (hypotensive) of intravenous bolus injections (6.4 µg/kg) of bradykinin and bradykinin azapeptides indicated by absolute change in mean arterial pressure (MAP) in rats. Each grey data point represents one animal. Mean values (with SEM where indicated) for animals given experimental agent were compared with vehicle. Asterisks denote p < 0.05 using a one-tailed T test. n = 2 animals for [azaF5, azaF8]-BK, *p = 0.0029; n = 2 animals for [azaP2, azaF8]-BK, *p = 0.0002; n = 3 animals for azaF8-BK, (*p = 0.002), n = 1 animal for azaG4-BK; n = 2 animals for azaP2-BK, *p = 0.0267; n = 2 animals for azaP3-BK, azaF5-BK, azaP7-BK and azaR9-BK; n = 2 animals for Bradykinin, *p = 0.0038.
