Fig. 7: WNT, BMP4, and RA signals modulation conditions PE/STM/PFH organoids specification and these organoids are key to generate a physiologically relevant epicardium-myocardium heart organoid model in vitro. | Nature Communications

Fig. 7: WNT, BMP4, and RA signals modulation conditions PE/STM/PFH organoids specification and these organoids are key to generate a physiologically relevant epicardium-myocardium heart organoid model in vitro.

From: Human multilineage pro-epicardium/foregut organoids support the development of an epicardium/myocardium organoid

Fig. 7: WNT, BMP4, and RA signals modulation conditions PE/STM/PFH organoids specification and these organoids are key to generate a physiologically relevant epicardium-myocardium heart organoid model in vitro.

a Progenitor cell composition of D5 aggregates obtained through temporal modulation of WNT signaling. b Structural organization and different cell populations present in PE/STM/PFH organoids highlighting the critical signaling pathways that should be activated during PE induction period (D5-D7) to generate those organoids. c Structural organization and different cell populations present in organoids generated through modulation of WNT, BMP4, and RA during PE induction period. Green spheres indicate activated signal and red spheres indicate not activated signal. d Co-culture process for the generation of EMOs, and identification of the different regions/cell populations present in EMOs. MZ myocardium-like zone, EL epicardial-like layer.

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