Fig. 6: Colocalization of cGAS to micronuclei in SSc fibroblasts.
From: Centromere defects, chromosome instability, and cGAS-STING activation in systemic sclerosis
IF analysis of expression of cGAS (red) in SSc fibroblasts. Nuclei and micronuclei were stained in DAPI (blue). a Micrographs showing cGAS expression and colocalization to micronuclei in an lcSSc patient (111). b Micrographs showing only cGAS expression but not co-localization to micronuclei in the same lcSSc patient (111). c Confirmation of the specificity of c-GAS staining in a and b. Representative fluorescence images and quantification using ImageJ showing reduction of cGAS expression in cGAS DsiRNA transfected lcSSc patient fibroblasts (111) compared to control DsiRNA transfected lcSSc patient fibroblasts (111). The scale bars are shown at the bottom right. Arrows indicate micronuclei. The bar graph shows the mean fluorescence level of cGAS expression in Control DsiRNA-treated cells vs cGAS DsiRNA-treated cells (n = 10 micrographs per experiment). ****p < 0.0001. Data are presented as mean values +/− SD. Source Data are provided as a Source Data file.
