Fig. 4: ZF-DdCBEs install pathogenic mutations in cultured cells in vitro.
From: Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivo
a The m.8340G>A mutation in human MT-TK disrupts the T-arm of mt-tRNALys. b Mitochondrial DNA base editing efficiencies of HEK293T cells treated with an optimized ZF-DdCBE pair designed to install m.8340G>A. c The m.7743G>A mutation in mouse Mt-tk disrupts the T-arm of mt-tRNALys. d Mitochondrial DNA base editing efficiencies of C2C12 cells treated with an optimized ZF-DdCBE pair designed to install m.7743G>A. e Mitochondrial DNA base editing efficiencies of C2C12 cells treated with an optimized ZF-DdCBE pair designed to install m.3177G>A. For (b, d and e), values and errors reflect the mean ± s.d. of n = 3 independent biological replicates. For each site the DNA spacing region, split DddA orientation, ZF array lengths, and ZF-targeted DNA strands (LT left top, LB left bottom, RB right bottom) are shown, and the cytosine with the highest editing efficiency is colored in blue. Source data are provided as a Source Data file.
