Fig. 1: Enzymatic incorporation of 4-SH-N-acetylglucosamine into the growing heparosan backbone to form a S-linked heparosan extension. | Nature Communications

Fig. 1: Enzymatic incorporation of 4-SH-N-acetylglucosamine into the growing heparosan backbone to form a S-linked heparosan extension.

From: Chemoenzymatic synthesis of sulfur-linked sugar polymers as heparanase inhibitors

Fig. 1

The heparosan synthase uses two sugar donors, UDP-GlcNAc or its 4-thiol analog and UDP-GlcA, to create the disaccharide repeats of the GAG backbone chain. (Yellow or red highlight boxes highlight critical sulfur or oxygen atoms in the glycosidic linkages, respectively; n = 1 to 2; m ~45; R = H, GlcNAc-GlcA-C2H4-NH2, GlcNAc-GlcA-C2H4-amido-benzaldehyde, or GlcNAc-GlcA-C2H4-thioamido-fluorescein as noted in Methods). Inset depicts the natural O-linked heparosan repeat for comparison.

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