Fig. 3: Characterization of GAC reporter at tissue levels.
From: A Glb1-2A-mCherry reporter monitors systemic aging and predicts lifespan in middle-aged mice
a Representative images showing mCherry signal (upper) and its co-localization with SAβ-gal staining (lower, mCherry in pseudo-color) in liver, spleen, lung, kidney and brain tissues from Glb1+/m mice (27 months, male, n = 3). Dashed polygon indicates area with intense senescence signal. Scale bar, 100 µm. b Left, representative images showing mCherry signal and anti-Lamin B1 staining in indicated tissue sections from Glb1+/m mice (23 months, female, n = 3). The magnified regions show cells with mutually exclusive signals for mCherry and Lamin B1. Right, quantification of mCherry and Lamin B1 signal intensity in indicated tissues (left). Over 100 cells per group were counted. Scale bar, 50 µm. “n” represents number of biological replicates. Data represent the means ± s.e.m. p value was calculated by Student’s t test (two-sided).
