Fig. 6: The METTL3-IGF2BP2-GYS2 axis is related to liver glycogen storage in rats.

a PAS staining of wild-type rat livers in different ages. The percentage of positive area is measured by Image J and shown in the right. Bar, 100 μm. n = 5 animals. Data are presented as mean values +/− SEM. Two-sided Student’s t test was performed to determine a difference among groups. ***p < 0.001. b Hepatic glycogen content assay of rat livers with indicated ages. n = 4 animals. Data are presented as mean values +/− SEM. Two-sided Student’s t test was performed to determine a difference among groups. ***p < 0.001. c The levels of m6A mRNA were analyzed in rat livers of different ages. n = 5 animals. Data are presented as mean values +/− SEM. Two-sided Student’s t test was performed to determine a difference among groups. ***p < 0.001. d, e qRT-PCR assay of Mettl3 (d) and Gys2 (e) in rats’ livers of different ages (β-actin as reference). n = 5 animals. Data are presented as mean values +/− SEM. Two-sided Student’s t test was performed to determine a difference among groups. **p = 0.002 in (d); ***p < 0.001. f Serum glucose level of indicated ages of rats in normal diet. n = 5 animals. Data are presented as mean values +/− SEM. Two-sided Student’s t test was performed to determine a difference among groups. **p = 0.005. Source data are provided as a Source data file.