Fig. 2: Blinking experiments.
From: Phosphorylation disrupts long-distance electron transport in cytochrome c
a Representative blinking recordings for Cc1-Cc (red) and Cc1-Y48pCMF Cc (blue). Blinks are shown as transient jumps of the current flowing between the two electrodes at a constant distance (imposed by initial current set point) and bias (800 mV). b Two-dimensional histograms of blink current (Iblink) and blink lifetime for Cc1-Cc (left, n = 100980 conductance values included) and for Cc1-Y48pCMF Cc (right, n = 252193 conductance values included). Counts have been normalized to a color scale, with 100 counts representing the maximum, and 0 representing the minimum. The corresponding conductance (G) profiles are shown. Baseline correction was applied in a and b. G = Iblink/Vbias is used to obtain the conductance values. G0 = 2e2/h = 77.5 μS, with h the Planck constant and e the elementary charge. c Plot of the averaged G values from gaussian fit of the most probable conductance peaks (mean ± s.d.) G = 4.3·10−6 ± 9·10−7 G0 for Cc1-Cc (red) and G = 7.4·10−6 ± 5·10−7 G0 for Cc1-Y48pCMF Cc (blue), respectively, showing significant differences (n = 6 conductance values from 2 independent experiments, two sample t-test, t Statistic = 2.777, degrees of freedom = 10.00, *P = 0.0195. d Plot of Iblink against its baseline current (Ibaseline) for a set of blinks of Cc1-Cc (red) and Cc1-Y48pCMF Cc (blue) (mean ± s.d.). The dashed vertical lines indicate the corresponding current set points at which feedback was turned off. Sample and probe electrodes are represented by a square and a triangle, respectively. All experiments were performed in 50 mM phosphate buffer, pH 6.5. Source data are provided as a Source Data file.
