Fig. 4: Comparison of G protein coupling of NMU2–Gi1 complex.
From: Ligand recognition and activation of neuromedin U receptor 2
a, b Comparison of the G protein binding pose in NMU2–Gi1, NST1–Gi (6OS9), CXCR2–Gi (6LFO), CCK1–Gi (7EZH), A1–Gi (6D9H) and CB1–Gi (6N4B) complexes. Gαi in NMU2–Gi1, NST1–Gi, CXCR2–Gi, CCK1–Gi, A1–Gi, and CB1–Gi complexes are shown as cartoon and colored by pale green, pale cyan, blue-white, pale yellow, light pink, and wheat. Gβ and Gγ are not displayed for clarity. Receptors are shown as a gray cartoon. Conformational changes of αN helix (a) and α5 helix (b) are indicated by red arrows. c Comparison of the α5 helix of Gi in the NMU2–Gi1 and other GPCR–Gi complexes. NMU2 is shown as a slate cartoon, and other receptors are shown as a gray cartoon. Conformational changes of the C-terminus of the α5 helix are indicated by red arrows. d–f Residues involved in the interactions between the α5 helix and the receptors are shown as sticks. Hydrogen bonds and salt bridges are indicated by green, and red dashed lines. g Comparison of the ICL2 in NMU2–Gi1 and other GPCRs–Gi complexes. The conformational change of ICL2 is indicated by red arrows. h–j Different binding modes between ICL2 of the receptor and G protein. NMU2 is shown as a slate cartoon, and other receptors are shown as a gray cartoon. Gs of β2AR-Gs complex is shown as a raspberry cartoon.
