Fig. 2: SWR1C evicts distal and proximal H2A from the nucleosome.
From: H2A.Z deposition by SWR1C involves multiple ATP-dependent steps
a–e Example trajectories. The Cy3B donor fluorophore was excited, and donor emission (green) and ATTO 647N acceptor emission (red) were recorded (top panel) and used to calculate energy transfer efficiency (blue, bottom panel). a Example trajectory of SWR1C evicting distal H2A (**) from the nucleosome followed by the eviction of proximal H2A (*). b Example trajectory of SWR1C evicting proximal H2A (*) from the nucleosome followed by the eviction of distal H2A (**). c Example trajectory of SWR1C eviction of H2A (#) from a nucleosome in a high energy transfer state. d Example trajectory of SWR1C eviction of H2A (#) from a nucleosome in a medium energy transfer state. e Example trajectory of SWR1C eviction of H2A (#) from the nucleosome in a low energy transfer state. f Observed events for each type of eviction (a–e) in the presence of 100 μM ATP (N = 939 from 8 replicates). Single eviction represents the summation of events c–e.
