Fig. 3: The duration of the priming and release phase is dependent on ATP concentration.
From: H2A.Z deposition by SWR1C involves multiple ATP-dependent steps
a Example trajectory highlighting priming, eviction, and release intervals. The Cy3B donor fluorophore was excited, and donor emission (green) and ATTO 647 N acceptor emission (red) were recorded (top panel) and used to calculate energy transfer efficiency (blue, bottom panel). The * indicates eviction of H2A. b Distribution of the priming phase duration for the indicated ATP concentration, 100 μM ATP N = 635 observed events for 939 nucleosomes from 8 replicates, 5 μM ATP N = 567 observed events for 940 nucleosomes from 8 replicates, 0.5 μM ATP N = 325 observed events for 865 nucleosomes from 7 replicates. c Distribution of the eviction phase duration for the indicated ATP concentration, 100 μM ATP N = 635 observed events for 939 nucleosomes from 8 replicates, 5 μM ATP N = 567 observed events for 940 nucleosomes from 8 replicates, 0.5 μM ATP N = 325 observed events for 865 nucleosomes from 7 replicates. e Distribution of the release phase duration for the indicated ATP concentration, 100 μM ATP N = 431 observed events for 939 nucleosomes from 8 replicates, 5 μM ATP N = 396 observed events for 940 nucleosomes from 8 replicates, 0.5 μM ATP N = 227 observed events for 865 nucleosomes from 7 replicates. In b–d black line is the median, c ns, not significant, for one-way ANOVA Tukey’s multiple comparison test. e Alignment of E.T. trajectories with eviction phase centered at zero with 100 μM ATP (N = 635 observed events for 939 nucleosomes from 8 replicates), red line is the median. Source data for (b) and (d) are provided with this manuscript.
