Fig. 3: Biophysical characterization of lipid binding to MsbA trapped with ADP and vanadate.
From: Structural basis for lipid and copper regulation of the ABC transporter MsbA
a Representative mass spectrum of MsbA trapped with ADP·VO4 and in the presence of 0.4 μM KDL. b Deconvolution of the mass spectrum shown in panel a. c Deconvoluted mass spectrum of non-trapped MsbA in the presence of the same amount of KDL. A significant reduction in KDL binding to MsbA is observed. d KD values for individual lipid binding events to MsbA trapped with ADP and vanadate. Reported are the mean and standard deviation (n=3, biological replicates). Source data are provided as a Source Data file.
