Table 1 Thermostability and kinetic properties of wild-type EUGO and mutants

From: Structure- and computational-aided engineering of an oxidase to produce isoeugenol from a lignin-derived compound

Variants

Tm (°C)

4-n-propylguaiacol

vanillyl alcohol

KM (µM)a

kcat (s−1)a

KM (µM)a

kcat (s−1)a

EUGO

66.5 ± 0.5

3.3 ± 0.9

0.008 ± 0.0003

43.0 ± 2.2

6.8 ± 0.2

S394V-EUGO5X

78.5 ± 0.0

3.4 ± 0.4

0.028 ± 0.0003

7.1 ± 0.6

0.075 ± 0.001

D151E/S394V-EUGO5X

76.5 ± 0.0

3.0 ± 0.2

0.080 ± 0.002

2.9 ± 0.2

0.15 ± 0.002

PROGO

81.6 ± 0.2

7.4 ± 0.4

0.43 ± 0.007

94.9 ± 7.8

2.8 ± 0.07

  1. aKM and kcat values are based on rates determined at 7 different substrate concentrations (each in duplicate); for each average, the error was smaller than 5%.
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