Fig. 5: S1P signaling via SREBP1 and PPARγ maintains membrane homeostasis in human/mouse cells.

A Average GP index from several images of WT MEFs treated with vehicle, betulin 1 μM (inhibitor of SREBP maturation) and PA 200 μM ± betulin 1 μM. n = 15 separate images analyzed for each condition. B Average GP index from several images of NT, AdipoR2 and SREBF1 siRNA HEK293 cells treated with PA 200 µM ± S1P 1 µM and ± OA 200 µM. For each condition from left to right, n = 10, 10, 10, 15, 15 and 10 separate images analyzed. C Western-Blot and quantification (n = 3 experiments) of NT and SREBF1 siRNA HEK293 cells treated with vehicle and PA 400 µM ± S1P 1 µM. The red arrow points to the precursor SREBP1 band. Note the presence of an unspecific band just below SREBP1 band. n = 3 biologically independent replicates per condition. Uncropped blots in Source Data. D, E Average GP index from several images of WT and R2KO MEFs treated with vehicle, PA (200 or 50 µM) ± T0070907 1 µM (PPARγ antagonist). In D, n = 10 separate images analyzed. In E, n = 15 for vehicle and 18 for T0070907. Representative images of D are shown in Supplementary Fig. 7A. F Average GP index from several images of NT and PPARG siRNA HEK293 cells treated with vehicle and PA 200 µM ± S1P 10 or 1 µM. n = 20 separate images analyzed for each condition. G Average GP index from several images of NT and RXRA siRNA HEK293 cells treated with vehicle and PA 200 μM ± S1P 1 μM. Data are represented as mean ± SEM. t-tests (two sided and assuming normality) were used to identify significant differences between treatments. *p < 0.05, **p < 0.01, ***p < 0.001. See also Supplementary Figs. 6, 7 and Supplementary Data 5. Source data are provided as a Source Data file.