Fig. 6: SCD is the final protein effector of the AdipoR2-S1P pathway. | Nature Communications

Fig. 6: SCD is the final protein effector of the AdipoR2-S1P pathway.

From: Sphingosine 1-phosphate mediates adiponectin receptor signaling essential for lipid homeostasis and embryogenesis

Fig. 6

A, B Western-Blot and quantification of WT and DKO MEFs in A and NT and AdipoR2/PPARγ/SCD siRNA in HEK293 cells in full media or basal media ± PA 200 µM. Uncropped blots in Source Data. C–F PA (16:0) and OA (18:1) abundance (mol%) and in the PC of HEK293 cells treated with different siRNA and PA 200 μM ± S1P 1 μM. For C and D, n = 4 independent biological replicates per condition. For E and F, n = 3 independent biological replicates per condition. Related lipidomics are shown in Supplementary Fig. 8M, N and in Data 6. G Average GP index from several images of NT and SCD siRNA HEK293 cells treated with vehicle and PA 200 µM ± S1P 10 µM and ± OA 200 µM. n = 15 separate images analyzed for each condition. Data are represented as mean ± SEM. t-tests (two sided and assuming normality) were used to identify significant differences between treatments. *p < 0.05, **p < 0.01, ***p < 0.001. See also Supplementary Fig. 8 and Supplementary Data 5–7. Source data are provided as a Source Data file.

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