Fig. 9: The p38α-BLIMP1 signal axis is required for LPS-induced PC generation.
From: A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation
a Naive B cells from the spleen of p38αfl/flVavcre or p38αfl/fl mice were stimulated with LPS for 4 days (n = 6 per group). The percentage of iPCs (CD19+CD138+) was analyzed by flow cytometry. b Cultured p38α KO and WT B cells were transduced with retroviruses encoding p38α or p38α kinase-dead mutants (K53M or T180A/Y182F) at day 1 of LPS-induced iPCs generation. iPCs among retrovirus-infected B cells (CD19+GFP+CD138+) were analyzed by flow cytometry 3 days after retroviral transduction (n = 6 per group). c Flow cytometry analysis of BLIMP1-expressing cells (CD19+GFP+), BLIMP1-expressing CD138− B cells (CD19+GFP+CD138−) and BLIMP1-expressing iPCs (CD19+GFP+CD138+) differentiated from cultured p38αfl/flVavcreBlimp1gfp/+ and p38αfl/flBlimp1gfp/+ B cells stimulated with LPS and MFI of GFP (indicating Blimp1 expression) in BLIMP1-expressing cells, GFP+CD138− B cells and iPCs (n = 4 per group). d Cultured p38α KO and WT B cells were transduced with retroviruses encoding BLIMP1, TCF3 (E12 or E47), TCF4, or IRF4 at day 1 of LPS-induced iPCs generation. iPCs among retrovirus-infected B cells (CD19+GFP+CD138+) were analyzed by flow cytometry 3 days after retroviral transduction (n = 6 per group). Data were representative of at least three independent experiments. Each symbol represents a representative sample. Small horizontal lines indicate the mean (±s.d.). Data were analyzed by two-tailed unpaired t-tests. Source data are provided as a Source Data file.
