Table. 1 Predicted K-serotype specificity and enzymatic activity of putative depolymerases of phage ΦKp24

From: High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers

Potential depolymerases

Potential serotype specificity

aPredicted enzymatic activity

bSimilar to

Accession number

Alignment with depolymerases of ϕKp24

Cover

E value

Percent identity

Identity range

gp168

-

pectate lyase, glycosidase

-

-

-

-

-

-

gp196

K2

pectate lyase, hydrolase

gp43 KLPN1

YP_009195383.1

85%

3E-176

50,18%

371/570

gp294

-

xylosidase, glycoside hydrolase

-

-

-

-

-

-

gp295

K1/K2/K57

xylosidase, pectin lyase-like

gp40 Henu1

YP_009818434.1

70%

4E-54

37%

152/494

gp300

K25

pectate lyase, hydrolase

gp59 K64-1

YP_009153199.1

99%

1E-153

42,38%

370/604

gp301

K35

pectate lyase, endo-N-acetylneuraminidases

gp60 K64-1

YP_009153200.1

70%

1E-105

33,03%

344/651

gp303

ND

pectate lyase, hydrolase

gp57 KpV48

YP_009787613.1

97%

8E-158

41,54%

275/662

gp304

-

pectate lyase, hydrolase

-

-

-

-

-

-

gp306

K1

pectate lyase, sugar binding protein, hydrolase

gp46 KpV41

YP_009188788.1

74%

3E-144

40,44%

381/680

gp307

ND

pectate lyase, glycoside hydrolase, lyase, sugar binding protein

gp45 phiKpS2

YP_009792400.1

78%

3E-75

34,53%

202/585

gp308

-

pectate lyase, hydrolase

-

-

-

-

-

-

gp309

KN4

sugar binding protein, hydrolase

gp18 KN4-1

YP_009818003.1

84%

2E-126

38,81%

248/639

gp310

-

pectate lyase, sugar binding protein, hydrolase

-

-

-

-

-

-

gp313

-

xylosidase, glycoside hydrolase, pectate lyase

-

-

-

-

-

-

  1. aThe predicted enzymatic activities of putative depolymerases were obtained using protein homology detection tools HMMER, HHPred, and Phyre2. ND no data available; no significant similarities were found.
  2. bPairwise alignment was performed on all potential ϕKp24 depolymerases using BlastP and alignment metrics with the best hit are shown.