Fig. 6: GPSM1 loss inhibits the pro-inflammatory signaling pathway via TNFAIP3.
From: GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages
a Venn diagram showing overlap of differentially expressed genes from two RNA-sequencing datasets. b Immunoblot analysis of A20 in BMDMs isolated from GPSM1f/f; Lyz2-cre and littermates following 200 ng/ml LPS treatment for indicated times. c Immunoblot analysis of p-IκBα, IκBα, p-P65, P65, and A20 in GPSM1f/f and GPSM1f/f; Lyz2-cre BMDMs infected with indicated lentivirus for 72 h and treated with LPS for additional indicated times. d–k Metabolic and inflammatory characterization of four genotypes mice fed a HFD for 10 weeks. d A20 and GPSM1 protein levels in BMDMs. e Body weight curve (left) and the body weight at 10-week HFD (right), n = 8 biologically independent mice for GPSM1f/fTNFAIP3f/+, Lyz2-cre; and n = 9 biologically independent mice for other three genotypes, respectively. f Tissue weights, including scWAT, eWAT, BAT, and Liver; n = 8 biologically independent mice for GPSM1f/fTNFAIP3;f/+ n = 7 biologically independent mice for other three genotypes, respectively. g GTT and AOC; n = 8 biologically independent mice for TNFAIP3f/+, Lyz2-cre; and n = 7 biologically independent mice for other three genotypes, respectively. h ITT and AOC; n = 8 biologically independent mice for TNFAIP3f/+, Lyz2-cre; n = 7 biologically independent mice for other three genotypes, respectively. i H&E (Scale bars, 50 μm) and F4/80+ IHC (Scale bars, 100 μm) staining of eWAT. j Representative flow cytometry analysis and quantification of the expression of CD11c from CD45+F4/80+ cells in SVFs from eWAT (n = 4 biologically independent mice per group). k RT-PCR analysis indicating mRNA abundance of pro-inflammatory and anti-inflammatory genes in eWAT, n = 8 biologically independent mice for GPSM1f/fTNFAIP3f/+ and GPSM1f/fTNFAIP3f/+, Lyz2-cre; n = 7 biologically independent mice for GPSM1f/f; Lyz2-cre and TNFAIP3f/+, Lyz2-cre. Throughout, all independent experiments were performed three times with similar results (b–d, i). Data are presented as means ± SEM. P values are determined by two-way ANOVA with Sidak’s multiple-comparisons test (e, g, and h) or one-way ANOVA with Tukey’s multiple-comparisons test (e–h, j, k). For e, g, and h, P values are compared with GPSM1f/fTNFAIP3f/+ group.
