Fig. 1: Experimental set-up for TR-SSX at 14-ID-B BioCARS beamline.

a Polychromatic x-ray beam depicted with simplified representation of pulse train exposure, containing 24 x-ray pulses with duration of 100 ps each and total pulse train duration of 3.7 µs. ALEX chip moves during scan in a meander-pattern over the preset area of the chip containing slurry of immobilized microcrystals. Laser pulse is coordinated with release of x-ray beam by a time delay that corresponds to a specific time point of reaction. The typical Laue diffraction pattern is shown obtained from L1 MBL crystal during TR-SSX experiment. b Movement of the ALEX chip across the Y-axis with step size labeled as blue bars with an overlaid red baseline that indicates the diameter of a laser pulse spot, source data is available as Source data file. c Scheme of fixed-target SSX data collection on nylon mesh crystals holder utilizing the pump-probe system. The X and Y axis step sizes of the chip are labeled as red arrows. SSX data collection were conducted as pump-probe system with specific time-delay between laser pulse and a train of 100 ps pink-beam x-ray pulses. To avoid the impact of zinc diffusion on data collection the step size of the SSX crystal holder was increased for the detection of longer time-points of moxalactam cleavage reaction. d Microscope photography of x-ray burn paper after test run of pump-probe SSX system.