Fig. 5: H3K27me3 occupancy in the KRT14 promoter is linked with active transcription.
From: EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
A Diagrammatic scheme showing the genomic location of the KRT14 gene for both mice and humans. B ChIP was performed in 4T-1 mouse cells using anti-H3K27me3, p-Pol-II-S5, and IgG antibodies and then examined by real-time qPCR using primer pairs targeting −1.5 to −0.2 kb of the KRT14 gene. C Same procedure for HCC1806 human cells as in (B). D, E The differential fold change enrichment for H3K27me3 and p-Pol-II-S5 were observed in −0.2 and −0.5 kb regions from TSS in control and EZH2-Y641-F cells by ChIP qPCR. F The differential fold change enrichment for H3K27me3 and p-Pol-II-S5 were analyzed in the −0.2 kb region from TSS in the HCC1806 cells treated either with vehicle control or 10 μM EPZ6438. G ChIP was performed in 4T-1 mouse cells using anti-H3K4me3 and IgG antibodies and then examined by real-time qPCR using primer pairs targeting −1.1 to +2.4 kb of the KRT14 gene. H Same procedure for HCC1806 human cells as in (G). I The differential fold change enrichment for H3K4me3 + 2.4 kb region from TSS in control and EZH2- Y641-F 4T-1 cells by ChIP qPCR. J The differential fold change enrichment for H3K4me3 was analyzed in +2.4 kb region from TSS in the HCC1806 cells treated either with vehicle control or 10 μM EPZ6438. B–F Columns, are the mean of quadruplicate readings of samples; error bars, ±S.D, B, C compared to IgG control, left to right, *P = 0.0158, #P = 0.0001, %P = 0.0003, $P = 0.0001, &P = 0.0001 and @P = 0.0004, two way ANOVA, Sidak’s multiple comparisons test. D–F compared to control, left to right, *P = 0.0029, #P = 0.0025, $P = 0.0022, &P = 0.0024, %P = 0.0036 and +P = 0.0134, two-way ANOVA, Turkey’s multiple comparisons test. G–I columns, a mean of duplicate readings of samples, error bars, ±S.D, G, H compared to IgG control, left to right, *P = 0.0023 and #P = 0.0001, two-way ANOVA, Sidak’s multiple comparison test. I compared to VC, *P = 0.0001, two-way ANOVA, Turkey’s multiple comparisons test. J Columns, a mean of triplicate readings of samples, error bars, ±S.D, compared to VC, #P = 0.0082, two-way ANOVA, Turkey’s multiple comparisons test. Source data are provided as a Source Data file.
