Fig. 4: Rad52’s N-terminal DNA binding domain is required for Rad51-independent template switching.

a Schematic showing the main interaction domains and conserved arginine-45 in fission yeast Rad52. The two C-terminal truncations, ∆C308 and ∆C208, are also shown. b Western blot showing the relative amounts of full length and truncated Rad52 in whole cell extracts from wild-type (MCW8888), rad52⁺-kanMX6 (MCW9694), rad52-R45A-kanMX6 (MCW9840), rad52∆C208-kanMX6 (MCW9692), rad52∆C308-kanMX6 (MCW9693) and rad52∆ (MCW1285) strains. Histone H3 serves as a loading control. An independent repeat of this experiment gave similar results. c Frequency of spontaneous (RTS1-IO) and RTS1-AO-induced Ade⁺ recombinants in rad52⁺ and rad52 C-terminal truncation mutant strains with ori1253∆ and the 12.4 kb reporter. The data are also reported in Supplementary Table 1, which includes the strain numbers, the number of colonies tested for each strain (n) and p values. Further details of the statistical analysis are reported in Supplementary Data 1. Source data are provided as a Source Data file.