Fig. 5: Rad52’s DNA annealing activity is required for RDR-associated template switching.

a Frequency of spontaneous (RTS1-IO) and RTS1-AO-induced Ade⁺ recombinants in ori-1253⁺ strains carrying the 12.4 kb reporter. b Frequency of spontaneous (RTS1-IO) and RTS1-AO-induced Ade⁺ recombinants in ori-1253∆ strains carrying the 12.4 kb reporter. c Frequency of spontaneous (RTS1-IO) and RTS1-AO-induced Ade⁺ recombinants in ori-1253⁺ strains carrying the 0.2 kb reporter. a–c Data are presented as median values ± interquartile range with individual data points shown as grey dots. The data are also reported in Supplementary Table 1, which includes the strain numbers, the number of colonies tested for each strain (n) and p values. Further details of the statistical analysis are reported in Supplementary Data 1. Source data are provided as a Source Data file. d Frequency of RTS1-AO-induced Ade⁺ recombinants in a rad52-R45A mutant relative to wild-type. Values are derived from the data in (a–c).