Fig. 2: Characterization of anti-CD1a antibodies in the inhibition of T-cell responses.

A Dose titration curve of polyclonal T-cell IFNγ response with increasing concentration of a refined panel of anti-CD1a antibody (0.01–10 µg/ml) as determined by ELISpot (n = 6 donors, mean ± SD). And table of half maximal inhibitory concentration (IC50) values (µg/ml) calculated for the panel of newly generated anti-CD1a antibodies and commercial antibodies (OKT6, HI149 and SK9). n = 6 biologically independent blood donors for all groups examined over 4 independent experiments. B–D Characterization of anti-CD1a antibodies in the inhibition of CD1a-restricted T cells. n represents biologically independent CD1a-restricted T-cell lines. B, C Cytokine secretion response of CD1a-restricted T cells induced by empty-vector (EV)- or CD1a-transfected (CD1a)-K562 presenting endogenous ligands. Inhibition of IFNγ (B) and IL-22 (C) was assessed for the panel of anti-CD1a antibodies by flow cytometry. D IFNγ secretion response of CD1a-restricted T cells induced by CD1a-coated beads presenting endogenous ligands. Inhibition was assessed for the panel of newly generated anti-CD1a antibodies by flow cytometry. B n = 9 examined over 4 independent experiments. C n = 4 examined over 2 independent experiments. D n = 19 examined over 4 independent experiments. One-way-ANOVA with Dunnett’s or Tukey’s test, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 where asterisk (*) indicates significance on comparison to “CD1a”, mean ± SEM). Exact p-values are recorded in Supplementary Table 2. Source Data are provided as a Source Data file.