Fig. 8: Analysis of the contribution of CD1a to MC903-induced inflammation.
From: CD1a promotes systemic manifestations of skin inflammation
A Schematic of MC903-induced inflammation with therapeutic anti-CD1a administration. B Daily measurement of ear swelling. n = 5 for all groups, two-way-ANOVA with Dunnett’s test, **P < 0.01; ****P < 0.0001 indicates significance on comparison to “CD1a” at day 7, examined over 2 independent experiments. C Representative images of inflammation (day 7), induced by MC903 treatment of wild-type (WT) and CD1a transgenic mice (CD1a) and countered by the treatment i.p. with mouse IgG1 isotype control or CD1a transgenic injected with the refined panel of anti-CD1a antibodies as in the schematic panel. D Flow cytometric analysis of ear skin of mouse IgG1 isotype treated wild-type (WT) and CD1a transgenic (CD1a) and CD1a transgenic injected with the refined panel of anti-CD1a antibodies. Skin T cells, neutrophils, eosinophils and LCs were enumerated (/ear). E Skin cytokine levels of mouse IgG1 isotype treated wild-type (WT) and CD1a transgenic (CD1a); and CD1a transgenic injected with anti-CD1a antibodies following the treatment model of administration. F Flow cytometric analysis of the blood of mouse IgG1 isotype treated wild-type (WT) and CD1a transgenic (CD1a) and CD1a transgenic injected with the refined panel of anti-CD1a antibodies. Skin T cells were enumerated and assessed for activation marker expression and blood neutrophil and, eosinophils were enumerated. G Plasma cytokine levels of mouse IgG1 isotype treated wild-type (WT) and CD1a transgenic (CD1a); and CD1a transgenic injected with anti-CD1a antibodies following the treatment model of administration as measured by cytometric bead array. D–G n = 7 (WT), n = 6 (CD1a, 16, 116), n = 5 (110), examined over 2 independent experiments. One-way-ANOVA with Dunnett’s test, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Mean ± SD is shown. n represents biologically independent animals in each group. Exact p-values are recorded in Supplementary Table 7. Source Data are provided as a Source Data file.
