Fig. 4: HTLV-1 specific Tax protein detected on the aGVHD skin tissues by IMC.

Representative IMC images of two independently stained skin tissues from 7 aGVHD patients show the overlap of Tax (red), DNA (blue) and different immune response markers. Displayed channels for each column were: A Tax (red)/DNA (blue); B CD4 (green)/Tax (red)/EIF1AY (white)/DNA (blue); C C4d (green)/Tax (red)/EIF1AY (white)/DNA (blue); D CD68 (green)/Tax (red)/EIF1AY (white)/DNA (blue). E Tax (red)/DNA (blue); F CD4 (green)/Tax (red)/DNA (blue); G C4d (green)/Tax (red)/DNA (blue); H CD68 (green)/Tax (red)/DNA (blue). Red arrows pinpoint the multi-signals overlap position and red boxes show the magnified view of CD4+T cells with Tax signal. Scale bar = 100 μm for landscape view and scale bar = 20 μm for magnified view.