Fig. 5: CD68+ mononuclear phagocyte (MNP) in skin sections of aGVHD patients. | Nature Communications

Fig. 5: CD68+ mononuclear phagocyte (MNP) in skin sections of aGVHD patients.

From: HTLV-1 infection of donor-derived T cells might promote acute graft-versus-host disease following liver transplantation

Fig. 5

Representative IMC images of two independently stained skin tissues from 3 aGVHD patients (ID139, 140, and 145) show the seven single signals of A DNA (blue), HLA-DR (red), CD11c (purple), CD14 (yellow), B CD123 (light blue), CD68 (green). C overlay signal of seven markers. The same markers are shown in images in each column. Red, yellow, green, and blue arrows, respectively, pinpoint the monocyte-derived macrophages (MDM), macrophages, plasmacytoid dendritic cells (pDC), and conventional dendritic cells (cDC). Red, yellow, green, and blue boxes respectively show the magnified multi-signal overlap position of MDMs, macrophages, pDC, and cDC. Scale bar = 100 μm for landscape view and scale bar = 20 μm for magnified view. D Each column is mononuclear phagocyte (MNP) identification in skin sections from each patient. Each row is the comprehensive phenotyping of one MNP, and the symbol+/− indicates the MNP is existence/absent in skin sections of each patient. Displayed channels for each row are: macrophage (CD14−CD11c−CD68+); MDM (CD14+CD11c−CD68+); cDC (CD11c+HLA-DR+CD68+); pDC (CD123+HLA-DR+CD68+).

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