Fig. 1: Human macrophages from rs8736 TT carriers demonstrate higher cytokine secretion upon TLR stimulation compared with C allele carriers. | Nature Communications

Fig. 1: Human macrophages from rs8736 TT carriers demonstrate higher cytokine secretion upon TLR stimulation compared with C allele carriers.

From: A metabolic associated fatty liver disease risk variant in MBOAT7 regulates toll like receptor induced outcomes

Fig. 1

a Scheme illustrating the experimental design. MDMs (n = 10/genotype) were treated for 24 h with the indicated TLRs ligands (Pam3Cys4 [TLR2], Poly(I:C) [TLR3], LPS [TLR4], and CpG [TLR9]). Shown is quantitative ELISA of the conditional media measuring b TNF-α (P = 0.04, 0.8, 0.02, and 0.9, respectively) and c MCP-1 (P = 0.02, 0.009, 0.04, and 0.01, respectively). d Radar chart representing fold increase of different chemokines and cytokines; values segregated by donor genotype secreted by MDMs (n = 7/genotype) were treated for 24 h with Pam3Cys4 [TLR2]. e Individual results of IL-1β, IL-8 and IL-33 (P = 0.0007, P = 0.01, P = 0.005, respectively). Data are represented by vertical bars and are mean ± sem; *P < 0.05, ** P < 0.01, ****P < 0.001. Statistical differences between groups were assessed by one-way ANOVA; multiple comparisons were corrected by Bonferroni correction. Source data are provided as a Source Data file.

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