Fig. 2: MBOAT7 is required for optimal secretion of cytokines and remodelling of phospholipids in MDMs. | Nature Communications

Fig. 2: MBOAT7 is required for optimal secretion of cytokines and remodelling of phospholipids in MDMs.

From: A metabolic associated fatty liver disease risk variant in MBOAT7 regulates toll like receptor induced outcomes

Fig. 2

Quantitative ELISA of the conditional media measuring TNF-α and MCP-1in Unstimulated MDMs and cells treated with the indicated TLRs ligands (Pam3Cys4 [TLR2], Poly(I:C) [TLR3], LPS [TLR4] and CpG [TLR9]) for 24 h in a MDMs (n = 4–5) were transfected with MBOAT7 siRNA or scramble as control, b MDMs (n = 4–6) were transfected with MBOAT7 overexpression plasmid or the empty vector as control. P-value <0.02 for all comparisons except for TLR3. c LC-MS/MS analysis of phospholipid species in MDMs transfected with MBOAT7 siRNA or scramble at base line and challenged with LPS for 24 h (n = 6). Statistical differences between groups were assessed by 2-tailed Student’s t test and the error bars indicate the mean ± sem; *P < 0.05, ** P < 0.01, ****P < 0.001. Box plots indicate the first and third quartiles with the median in between, the whiskers extend to the furthest point within 1.5× the inter-quartile range past the corresponding quartile. Source data are provided as a Source Data file.

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