Fig. 5: GPR174 deficiency mitigates the inflammation in ischemic muscles through AREG.
a Representative flow cytometric dot plots of leukocyte populations in gastrocnemius tissues of WT and Gpr174−/Y mice 7 days after HLI. b Quantification of all leukocytes, neutrophils, total macrophages, Ly6Clow macrophages, and Ly6Chigh macrophages in WT and GPR174-deficient gastrocnemius 7 days post HLI (n = 7 for WT mice; n = 8 for Gpr174−/Y mice). c Scheme of Tregs-macrophages co-culture. d Relative mRNA levels of proinflammatory (upper panel) and anti-inflammatory (lower panel) genes in macrophages co-cultured with Tregs and AREG neutralizing antibodies for 24 h (n = 4). e Quantification of bioactive TGF-β and VEGF in macrophages stimulated with recombinant AREG in the presence or absence of inhibitors for the EGFR (Gefitinib) for 24 h (n = 8). For all statistical plots, the data are presented as mean ± SD. Two-tailed unpaired t-tests in (b). Two-way ANOVA with Bonferroni multiple comparisons test in (d, e). Source data are provided as a Source Data file.
