Fig. 1: Identification of a Lyve1+CX3CR1– population in the mouse brain.
From: Deciphering the heterogeneity of the Lyve1+ perivascular macrophages in the mouse brain
Confocal microscopy of brain sections stained with anti-Lyve1 showing the different Lyve1+ cell morphologies in the cortex at the dorsal side (17 µm maximum intensity projection) (a), in the cortex at the ventral side (27 µm maximum intensity projection) (b), the Hippocampal fissure within the hippocampus (15 µm maximum intensity projection) (c), and in the olfactory bulb (61 µm maximum intensity projection) (d) of an adult mouse brain. e–i Immunofluorescence microscopy on sections of Cx3cr1GFP in the dorsal cortex of the mouse brain. e Staining of Cx3cr1GFP sections for Lyve1 (red) and F4/80 (white) (50 µm maximum intensity projection), f Iba1 (29 µm maximum intensity projection), g CD206 (20 µm maximum intensity projection), h CD45 (46 µm maximum intensity projection), i MHC-II (18 µm maximum intensity projection). White arrows point to conventional Lyve1+CX3CR1+ pvMs. The red arrows indicate Lyve1+CX3CR1– cells. The pink arrows indicate Lyve1−MHCII+ cells. (n = 4).
