Fig. 5: The origin of CX3CR1^– Lyve1⁺ perivascular macrophages.

a Immunofluorescence on sections of the head of a E18.5 Spi1^GFP/+ embryo (PU1-GFP in green), labeled for Lyve1 (red) and CD45 (white) (60 µm maximum intensity projection) shows the presence of the conventional (white arrows) and the non-conventional pvM populations (red arrows) at this stage (n = 3). b No GFP nor Lyve1 fluorescence was observed in the Spi1^GFP/GFP E18.5 dorsal cortex (33 µm maximum intensity projection) (n = 2). c Immunofluorescence on Spi1^GFP/+ adult mouse brain (48 µm maximum intensity projection) (n = 3). d Immunofluorescence on sections of Cx3cr1^GFP/+; Cx3cr1-Cre; Rosa26^tdT mouse brain showing that conventional (white arrows) and non-conventional pvM population (red arrows) are expressing tdTomato (n = 3). e Flow cytometry analysis of Cxcr4-CreErt2; Rosa26^tdT brain parenchyma, gated on living, single cells and CD64⁺ F4/80⁺ CD206⁺ cells. Subsequently histograms of CD45 and Lyve1 expression of the CX3CR1^–tdTomato^– population in blue, the CX3CR1⁺tdTomato⁻ population in red and FMO in gray (n = 3). Source data are provided as a Source Data file.