Fig. 1: IL6 promotes primary hepatocytes expansion in vitro.
From: IL6 supports long-term expansion of hepatocytes in vitro
a Schematic of IL6-induced hepatocyte to iHPC reprogramming and subsequent maturation to iMHs. b Representative images of the primary hepatocytes (Hepa, D0) and hepatocytes cultured with or without IL6 for 14 days (D14) (repeated for three times). c Growth curves of primary hepatocytes cultured with or without IL6 for 14 days. Data are Means ± SEM (n = 3 independent experiments). ***p < 0.001 (two-way ANOVA). Immunofluorescence staining of cell cycle markers CyclinD1 (red) and Ki67 (green), HPC markers Sox9 (red) and Ck19 (green) (d) and hepatic markers Hnf4α (red) Albumin (green), Cyp1a2 (red) and Cyp2c9 (green) (e) in primary hepatocytes (Hepa), IL6-iHPCs (D14) and IL6-iMHs (D21). Nuclei were stained with Hoechst 33342 (blue). f Statistical data of the immunofluorescence staining data in d and e. Data are Means ± SEM (Cells from three mice were analyzed independently, three random fields/mouse). *p < 0.05, ***p < 0.001 (two-tailed unpaired Student’s t test). g Representative images of PAS staining and ICG uptake in primary hepatocytes and IL6-iMHs (D21) (repeated for three times). Scale bars represent 100 µm. DEX dexamethasone, OSM oncostatin M. Source data and the exact p values are provided in a Source data file.
