Fig. 3: Generation of iHPC clones from single cells with IL6.
From: IL6 supports long-term expansion of hepatocytes in vitro
a Schematic diagram of the genetic fate mapping method used to produce mice with hepatocytes expressing tdTomato (td-Hepa). b Representative morphologies and tdTomato (red) images of td-Hepa and three IL6-td-iMH clones (P10) derived from single cells. c The albumin and urea secretion capacities of IL6-td-iMH clones (P10) and primary td-Hepa. Data are Means ± SEM (n = 4 independent experiments). Representative PAS staining, ICG uptake (d) and immunofluorescence staining of hepatic markers Albumin (green) and Hnf4α (green) (e) in primary td-Hepa and IL6-td-iMH clones (P10). Nuclei were stained with Hoechst 33342 (blue). f Statistical data of the immunofluorescence staining data in e. Data are Means ± SEM (eight random fields for each group, three independently generated IL6-td-iMH clones were analyzed). g Transcriptome analysis among primary hepatocytes, IL6-iHPCs-D3 (P0), IL6-iHPCs-D14 (P0), IL6-iHPCs from different mouse and different passages and the IL6-iMHs differentiated from them (n = 3 technical repeats). The color bar at the right indicates gene expression in log2 scale. Red and green represent higher and lower gene expression levels respectively. h Pearson correlation map of primary hepatocytes, IL6-iHPCs-D3 (P0), IL6-iHPCs-D14 (P0), IL6-iHPCs from different mouse and different passages and the IL6-iMHs differentiated from them. The color bar at the right indicates a value of correlation between two samples. Red and blue colors represent higher or lower correlation coefficient between samples (n = 3 technical repeats). Scale bars represent 100 µm. Source data are provided in a Source data file.
