Fig. 1: Ligand dependent cell spreading and locomotion.
From: Single-molecule characterization of subtype-specific β1 integrin mechanics
a Schematic of TGT presenting a ligand for integrins. TGT-54pN (Cy3) immobilized on PEG-passivated surface is depicted. b Representative reflection interference contrast microscopy (RICM) images of cells seeded (1 h) on surfaces with immobilized TGT or adsorbed biological ligands. Scale, 10 µm. c Percentage of spread cells on LDVP-54pN or RGD-54pN surfaces (mean ± SD; n = 3 independent experiments). Cells were seeded with soluble ligands (LDVP or RGD peptides, 100 µM) in solution. d Close-contact enclosed areas from RICM images (mean ± SE; n = 43, 64, 96, 59, 50, 41 cells). e Aspect ratio of the enclosed areas (mean ± SE; n = 33, 24, 32, 56, 36, 33 cells, >500 μm2). f Cell morphology change and locomotion over time. Hoechst signals (cyan) overlaid on differential interference contrast (DIC) images (gray) show nuclei. Scale, 50 µm. g Cell nucleus trajectories (2–5 h after seeding) on RGD-54pN surface. h Mean square displacement (n = 114 (RGD-54pN) and 164 (LDVP-54pN) cells, mean ± SD). The red line shows the fitted power-law curve. Two-sided t-test for p-values. All results are representative of multiple experiments. Data are combined from three independent experiments. Source data are provided as a Source Data file.
